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TP0100
Sigma
Total Protein Kit, Micro
Description
| Testing Method | Colorimetric, Endpoint |
| Principle | Brilliant blue G (Coomassie blue) reacts with protein in an acid-alcohol medium to form a blue-colored protein dye complex. The color, measured at 595 nm, is proportional to the protein concentration. |
| Suitability | Suitable for protein determination for samples of up to 0.5 mg/ml. |
Properties
| test parameters | dynamic range: 50 mg/dL, 0.5 g/L |
| sample volume: 50 μL | |
| temp.: 18-26 °C | |
| total test time: 2 min | |
| wavelength: 595 nm | |
| working reagent stability 18-26°C: 2 weeks |
Safety
| Hazard Codes | T,B |
| Risk Statements | 23/24/25-34-39/23/24/25 |
| Safety Statements | 7-16-26-36/37/39-45 |
| RIDADR | UN 2924 9/PG 3 |
Components
| Kit component only | Protein Standard 5ml |
| Brilliant Blue Protein Reagent 120ml |
